CURRICULUM VITAE SILVIA ROCCHICCIOLI • Personal Information SILVIA ROCCHICCIOLI, Via Liguria 4, 56124, Pisa, Italy (home address) and Via Moruzzi 1, 56124, Pisa, Italy (work address) (+39) 050 3153093 Mobile: (+39) 328 0060686 silvia.rocchiccioli@ gmail.com; silvia.rocchiccioli@ifc.cnr.it Date of Birth: 14 May1979 Researcher unique identifier: ORCID Id 0000-0003-3831-4200 URL for web site: https://www.ifc.cnr.it/index.php/it/biologia-preclinica/proteomica-e-system-biology • Academic age and positions INSTITUTION DEGREE FROM YEAR University of Pisa, Italy TO YEAR Master degree in Chemistry 1998 University of Pisa, Italy 2003 PhD in Chemistry 2003 IFC-CNR 2007 Post-doc Fellow 2007 IFC-CNR 2012 Fixed time Researcher 2012 IFC-CNR 2017 Permanent Researcher 2017 current Dr. Silvia Rocchiccioli is a permanent CNR Researcher with Ph.D. in Chemist, Head of Proteomics and Lipidomics Laboratory at IFC-CNR (Pisa). Dr. Silvia Rocchiccioli graduated in 2003 in Chemistry at the University of Pisa, where she took her PhD in Chemical Sciences in February 2007. Since June 2007 she is working on proteomic/lipidomic analysis and evaluation of mass spectrometry data at the Institute of Clinical Physiology of CNR. In June 2012 she was enrolled as term-fixed Researcher and Team Leader of Proteomics and Lipidomics Laboratory at IFC-CNR (Pisa). • Teaching activities and PhD supervision 2012 Teaching assistant for Course of Advanced Bioinformatics (UNIPI). 2022 Teaching assistant for Course of Functional Biochemistry (UNIPI) Supervisor of: PhD Degree-2017- (UNIPI) “CLINICAL/MOLECULAR FEATURES AND PROTEOMICS DATA TO IDENTIFY DETERMINANTS OF CAROTID PLAQUE RISK” (etd-03242017-221649) Master Degree-2019- UNIPI- ANALISI PROTEOMICA SU VESCICOLE EXTRACELLULARI PER L’IDENTIFICAZIONE DI BIOMARCATORI DI SINDROME DI SJOGREN (etd-02142019-114904) Master Degree-2020- (UNIPI)- “Studio pilota del rischio clinico nella malattia aterosclerotica carotidea mediante un approccio anatomo-funzionale multi-imaging della placca” (etd-02202020-174506) Master Degree-2020- (UNIPI)- Proteomics signature of hepatocarcinoma cells throughout the extracellular vesicles analysis (etd-09012020-181759) PhD Degree-2022- (UNIPI)- Evaluation of radiomic features of carotid atherosclerotic plaques from CT angiography and integration with post-endarterectomy micro-CT imaging (etd-06292022-153541) • Other work experience (e.g. consultancy) Co-Promoter of four Clinical Protocols/Trials: ATEROMARK – Studi proteomici e molecolari per la diagnosi della vulnerabilità della placca aterosclerotica e correlazioni con tecniche di immagine- Protocol number 67725 22/11/2012 Comitato Etico Area Vasta Nord Ovest (2012-2023); SMARTool Clinical Study Protocol Number 23524- 14/04/2016 Comitato Etico Area Vasta Nord Ovest (2016-2021), SMARCLI-“Simulation Modeling of coronary ARtery disease: CLInical evaluation of a decision support system developed in H2020-SMARTool project” Protocol number 18474 -22/10/2020 Comitato Etico Area Vasta Nord Ovest (2020-2025), • Scientific organizations/Coordination of academic activities Dr. Silvia Rocchiccioli is responsible of a completely equipped laboratory for chromatographic separation and mass spectrometry analysis of biological samples. Major equipment of laboratory includes Ultra-centrifuge and Centrifuges Beckman Coulter, Western Blot and gel-free proteomics studies (HPLCs and mass spectrometers), one analytical- and one micro-HPLC system (Shimadzu Nexera and LC200-Eksigent), and 2 mass spectrometers (SCIEX): 5600TripleTOF for high-throughput profiling/high-resolution analysis/comparative semi-quantitative analysis; 5500QTRAP for quantitation and targeted analysis. The research activity of Dr. Silvia Rocchiccioli is focused on the proteomics and lipidomics approaches to evaluate biomarkers of cardiovascular/cardiometabolic and neurodegenerative diseases in plasma/serum, cellular extracts and tissues from explants. These approaches have been successfully applied in different contexts, spanning from in vitro cell models to animal models and clinical trials. Dr. Silvia Rocchiccioli is developing new approaches for extracellular vesicle, lipids and peptide fractionation and identification targeted to the non-invasive discovery and validation of molecular biomarkers. Based on the proteomic and lipidomic profiling of biological samples, the research mainly aims at disclosing molecular mechanisms and factors involved in onset and progression of diseases. • Fundings (main projects) FUND BY YEAR TITLE POSITI ON SOURCE WEBSITE Ministry of Health with PNRR: M6/C2_CA LL 2022 20222025 Health improvements by Understanding Residual Risk In CAd and NEw targets for prevention/treatment – (HURRICANE) PI https://www.salute.gov.it/portale/ ministro/p4_10_1_1_atti_1_1.jsp ?lingua=italiano&id=295 Regione Toscana 20202023 BANDO RICERCA SALUTE 2018- Regione Toscana DEM-AGING Neurodegenerative disorders throughout the lifespan. Autophagydependent biomarkers for trial readiness from infantile neuronal ceroid-lipofuscinoses to senile dementias PI https://www.demaging.net/ EU Commissio n 20162019 Horizon2020-SMARTool “Simulation Modeling of coronary ARTery disease: a tool for clinical decision support” GRANT AGREEMENT 689068 EU Coordin ator http://www.smartool.eu/ EU Commissio n 20172020 H2020-InSilC-P777119 In-silico trials for drug eluting BVS design, development and evaluation PI https://insilc.eu/ EU Commissio n 20082013 FP7 project ARTREAT-PN224297- Multi-level patient-specific artery and atherogenesis model for outcome prediction, decision support treatment, and virtual hand-on training WP Leader http://www.artreat.org/ • Bibliometric data H-Index (in Scopus): 22 Total number of publications in peer-reviewed journals N=131 Some examples of publications: Title Authors Source Title Publication Year DOI Proteomics and lipidomic analysis reveal dysregulated pathways Daniele Galatolo†, Silvia Rocchiccioli†, Nicoletta Di Giorgi, Frontiers Neuroscience 2024 10.3389/fnins.2024.1375299 associated with loss of sacsin Flavio Dal Canto, Giovanni Signore, Federica Morani, Elisa Ceccherini, Stefano Doccini, Filippo Maria Santorelli Plasma Lipidomics and Coronary Plaque Changes: A Substudy of the SMARTool Clinical Trial Jeff M Smit, Silvia Rocchiccioli* , Giovanni Signore , Elena Michelucci, Nicoletta Di Giorgi, Alexander R van Rosendael , Mohammed El Mahdiui , Danilo Neglia, Juhani Knuuti , Antti Saraste , Ronny R Buechel , Anna Teresinska , Maria N Pizzi , Albert Roque , Rosa Poddighe , Bart J Mertens , Chiara Caselli, Oberdan Parodi , Gualtiero Pelosi , Arthur J Scholte* European Heart JournalCardiovascular Imaging 2024 10.1093/ehjci/jeae058 Proteomic Modulation in TGF-β-Treated Cholangiocytes Induced by Curcumin Nanoparticles Elisa Ceccherini, Giovanni Signore , Lorena Tedeschi , Federico Vozzi , Nicoletta Di Giorgi, Elena Michelucci AntonellaCecchettini , Silvia Rocchiccioli INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2023 10.3390/ijms241310481 Ceramides as Mediators of Oxidative Stress and Inflammation in Cardiometabolic Disease Gaggini, Melania; Ndreu, Rudina; Michelucci, Elena; Rocchiccioli, Silvia; Vassalle, Cristina INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2022 10.3390/ijms23052719 Machine Learning Coronary Artery Disease Prediction Based on Imaging and Non-Imaging Data Kigka, Vassiliki I.; Georga, Eleni; Tsakanikas, Vassilis; Kyriakidis, Savvas; Tsompou, Panagiota; Siogkas, Panagiotis; Michalis, Lampros K.; Naka, Katerina K.; Neglia, Danilo; Rocchiccioli, Silvia; Pelosi, Gualtiero; Fotiadis, Dimitrios I.; Sakellarios, Antonis DIAGNOSTICS 2022 10.3390/diagnostics12061466 Blood M2-like Monocyte Polarization Is Associated with Calcific Plaque Phenotype in Stable Coronary Artery Disease: A Sub-Study of SMARToo1 Clinical Trial Sbrana, Silverio; Cecchettini, Antonella; Bastiani, Luca; Di Giorgi, Nicoletta; Mazzone, Annamaria; Ceccherini, Elisa; Vozzi, Federico; Caselli, Chiara; Neglia, Danilo; Clemente, Alberto; Scholte, Arthur J. H. A.; Parodi, Oberdan; Pelosi, Gualtiero; Rocchiccioli, Silvia BIOMEDICINES 2022 10.3390/biomedicines10030565 Non-Invasive Prediction of Site-Specific Coronary Atherosclerotic Plaque Progression using Lipidomics, Blood Flow, and LDL Transport Modeling Sakellarios, Antonis I.; Tsompou, Panagiota; Kigka, Vassiliki; Siogkas, Panagiotis; Kyriakidis, Savvas; Tachos, Nikolaos; Karanasiou, Georgia; Scholte, Arthur; Clemente, Alberto; Neglia, Danilo; Parodi, Oberdan; Knuuti, Juhani; Michalis, Lampros K.; Pelosi, Gualtiero; Rocchiccioli, Silvia; Fotiadis, Dimitrios I. APPLIED SCIENCESBASEL 2021 10.3390/app11051976 Lipid biomarkers in statin users with coronary artery disease annotated by coronary computed tomography angiography Michelucci, Elena; Di Giorgi, Nicoletta; Finamore, Francesco; Smit, Jeff M.; Scholte, Arthur J. H. A.; Signore, Giovanni; Rocchiccioli, Silvia SCIENTIFIC REPORTS 2021 10.1038/s41598-021-92339-0 A specific plasma lipid signature associated with high triglycerides and low HDL cholesterol identifies residual CAD risk in patients with chronic coronary syndrome Di Giorgi, Nicoletta; Michelucci, Elena; Smit, Jeff M.; Scholte, Arthur J. H. A.; El Mahdiui, Mohammed; Knuuti, Juhani; Buechel, Ronny R.; Teresinska, Anna; Pizzi, Maria N.; Roque, Albert; Poddighe, Rosa; Parodi, Oberdan; Pelosi, Gualtiero; Caselli, Chiara; Neglia, Danilo; Rocchiccioli, Silvia ATHEROSCLEROSIS 2021 10.1016/j.atherosclerosis.2021.11.013 SmartFFR, a New Functional Index of Coronary Stenosis: Comparison With Invasive FFR Data Siogkas, Panagiotis K.; Lakkas, Lampros; Sakellarios, Antonis, I; Rigas, George; Kyriakidis, Savvas; Stefanou, Kostas A.; Anagnostopoulos, Constantinos D.; Clemente, Alberto; Rocchiccioli, Silvia; Pelosi, Gualtiero; Parodi, Oberdan; Papafaklis, Michail, I; Naka, Katerina K.; Michalis, Lampros K.; Neglia, Danilo; Fotiadis, Dimitrios, I FRONTIERS IN CARDIOVASCULAR MEDICINE 2021 10.3389/fcvm.2021.714471 Predictive Added Value of Selected Plasma Lipids to a Reestimated Minimal Risk Tool Bodini, Antonella; Michelucci, Elena; Di Giorgi, Nicoletta; Caselli, Chiara; Signore, Giovanni; Neglia, Danilo; Smit, Jeff M.; Scholte, Arthur J. H. A.; Mincarone, Pierpaolo; Leo, Carlo G.; Pelosi, FRONTIERS IN CARDIOVASCULAR MEDICINE 2021 10.3389/fcvm.2021.682785 Gualtiero; Rocchiccioli, Silvia Maternal Obesity and Cardiac Development in the Offspring Study in Human Neonates and Minipigs Guzzardi, Maria Angela; Liistro, Tiziana; Gargani, Luna; Ali, Lamia Ait; D’Angelo, Gennaro; Rocchiccioli, Silvia; La Rosa, Federica; Kemeny, Alessandra; Sanguinetti, Elena; Ucciferri, Nadia; De Simone, Mariarosaria; Bartoli, Antonietta; Festa, Pierluigi; Salvadori, Piero A.; Burchielli, Silvia; Sicari, Rosa; Iozzo, Patricia JACCCARDIOVASCULAR IMAGING 2018 10.1016/j.jcmg.2017.08.024 Hypothesis-free secretome analysis of thoracic aortic aneurysm reinforces the central role of TGF-beta cascade in patients with bicuspid aortic valve Rocchiccioli, Silvia; Cecchettini, Antonella; Panesi, Paola; Farneti, Pier Andrea; Mariani, Massimiliano; Ucciferri, Nadia; Citti, Lorenzo; Andreassi, Maria Grazia; Foffa, Ilenia JOURNAL OF CARDIOLOGY 2017 10.1016/j.jjcc.2016.05.007 Differential proteome profile in ischemic heart disease: Prognostic value in chronic angina versus myocardial infarction. A proof of concept Scebba, Francesca; Papale, Massimo; Rocchiccioli, Silvia; Ucciferri, Nadia; Bigazzi, Federico; Sampietro, Tiziana; Carpeggiani, Clara; L’Abbate, Antonio; Coceani, Flavio; Angeloni, Debora CLINICA CHIMICA ACTA 2017 10.1016/j.cca.2017.05.010 Extracellular matrix characterization in plaques from carotid endarterectomy by a proteomics approach Ucciferri, N.; Rocchiccioli, S.; Comelli, L.; Marconi, M.; Ferrari, M.; Pelosi, G.; Cecchettini, A. TALANTA 2017 10.1016/j.talanta.2017.06.014 Inflammatory and Antioxidant Pattern Unbalance in ClopidogrelResistant Patients during Acute Coronary Syndrome Caruso, Raffaele; Rocchiccioli, Silvia; Gori, Anna Maria; Cecchettini, Antonella; Giusti, Betti; Parodi, Guido; Cozzi, Lorena; Marcucci, Rossella; Parolini, Marina; Romagnuolo, Ilaria; Citti, Lorenzo; Abbate, Rosanna; Parodi, Oberdan MEDIATORS OF INFLAMMATION 2015 10.1155/2015/710123 Low T3 State Is Correlated with Cardiac Mitochondrial Impairments after Ischemia Reperfusion Injury: Evidence from a Proteomic Approach Forini, Francesca; Ucciferri, Nadia; Kusmic, Claudia; Nicolini, Giuseppina; Cecchettini, Antonella; Rocchiccioli, Silvia; Citti, Lorenzo; Iervasi, Giorgio INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2015 10.3390/ijms161125973 Characterization of secreted vesicles from vascular smooth muscle cells Comelli, Laura; Rocchiccioli, Silvia; Smirni, Salvatore; Salvetti, Alessandra; Signore, Giovanni; Citti, Lorenzo; Trivella, Maria Giovanna; Cecchettini, Antonella MOLECULAR BIOSYSTEMS 2014 10.1039/c3mb70544g Secreted proteins from carotid endarterectomy: an untargeted approach to disclose molecular clues of plaque progression Rocchiccioli, Silvia; Pelosi, Gualtiero; Rosini, Silvia; Marconi, Michele; Viglione, Federica; Citti, Lorenzo; Ferrari, Mauro; Trivella, Maria Giovanna; Cecchettini, Antonella JOURNAL OF TRANSLATIONAL MEDICINE 2013 10.1186/1479-5876-11-260 Correlation between vitamin D binding protein expression and angiographicproven coronary artery disease Rocchiccioli, Silvia; Andreassi, Maria G.; Cecchettini, Antonella; Carpeggiani, Clara; Abbate, Antonio L’; Citti, Lorenzo CORONARY ARTERY DISEASE 2012 10.1097/MCA.0b013e328358781c Proteomics changes in adhesion molecules: a driving force for vascular smooth muscle cell phenotypic switch Rocchiccioli, Silvia; Ucciferri, Nadia; Comelli, Laura; Trivella, Maria Giovanna; Citti, Lorenzo; Cecchettini, Antonella MOLECULAR BIOSYSTEMS 2012 10.1039/c2mb05470a Data: 29/01/2025 FIRMA Autorizzo il trattamento dei miei dati personali presenti nel CV ai sensi dell’art. 13 del Decreto Legislativo 30 giugno 2003, n. 196 “Codice in materia di protezione dei dati personali” e dell’art. 13 del GDPR (Regolamento UE 2016/679).